About half of the cells in the human brain are not neurons. Our aim is to understand the contribution of glial cells and particularly that of cortical astrocytes and Bergmann glia of the cerebellum to brain metabolism and signaling. We focus on how ion channels, transporters and organelles of the glial perisynaptic processes ensheathing neuronal synapses contribute to shaping and modulating synaptic transmission. Members of the ‘Biophysics of the Brain’ team work together to develop new optical tools for super-resolution and 3-D tissue imaging of glial morphology and physiology. We use various linear and non-linear microscopy techniques together with immunofluorescence, electrophysiology and advanced mouse genetics to crack the astrocyte calcium code. Our biological preparations range from cell culture, brain slices and hiPSC-derived 3-D organoids to mice, in vivo. A new line of research focusses on tissue clarification and 1- and 2-photon light-sheet imaging.
Team leader
Martin Oheim, Director of SPPIN, Senior Research Scientist, PI, CNRS, Core unit, Team 1 - Biophysics of the brain, +33 1 70 64 99 14 (secretariat), room E360
Members
Vincent Armand, Assistant Professor, Université Paris Cité, Team 1 - Biophysics of the brain, +33 1 42 86 42 21, room P368
Carine Julien, Assistant Professor - ENS Cachan, Team 1 - Biophysics of the brain, +33 1 42 86 42 21, room P368
Clément Ricard, Assistant Professor, Université Paris Cité, Team 1 - Biophysics of the brain, room H344
Adi Salomon, Chateaubriand Senior Research Fellow, Team 1 - Biophysics of the brain, +33 1 76 53 42 11, room H353
Recent Publications
Articles
- The gut, a whistleblower, in the early stages of Parkinson’s disease
Doriane Hazart, Malvyne Rolli-Derkinderen, Brigitte Delhomme, Pascal Derkinderen, Martin Oheim, Clément Ricard : Médecine/sciences, 2024 - Characterizing human astrocyte calcium signaling in a cerebral organoid
Kaitlin Szederkenyi, Liliana Attisano, Martin Oheim, Christopher M Yip : Biophys J, 2024 - L’intestin, lanceur d’alerte, dans les prémices de la maladie de Parkinson
Doriane Hazart, Malvyne Rolli-Derkinderen, Brigitte Delhomme, Pascal Derkinderen, Martin Oheim, Clément Ricard : Médecine/sciences, 2024 - Complementary Speckle STED Microscopy
Payvand Arjmand, Samlan Chandran Thodika, Elsa Bivas, Haoyang Li, Martin Oheim, Hiroyuki Yoshida, Etienne Brasselet, Marc Guillon : (preprint), 2024 - A colour-encoded nanometric ruler for axial super-resolution microscopies
Ilya Olevsko, Omer Shavit, Moshe Feldberg, Yossi Abulafia, Adi Salomon, Martin Oheim : Optics Communications, 2024 - Morphological Heterogeneity of Astrocytes in a Cerebral Organoid
K Szederkenyi, L Attisano, M Oheim, CM Yip : Glia, 2023 - Methods and applications in frontiers in neuroanatomy
Clément Ricard, Lidia Alonso-Nanclares, Basilis Zikopoulos, Martin Oheim : Front Neuroanat, 2023 - Characterizing nanometric thin films with far-field light
Klimovsky H, Shavit O, Julien C, Olevsko I, Hamode M, Abulafia Y, Suaudeau H, Armand V, Oheim M, Salomon A : Adv Opt Mater, 2023 - Nanoscopy of Aluminum Plasmonic Cavities by Cathodoluminescence and Second Harmonic Generation
Zar T, Ron R, Shavit O, Krause A, Gachet D, Salomon A : Advanced Photonics Research, 2022 - Label-free, fast, 2-photon volume imaging of the organization of neurons and glia in the enteric nervous system.
Hazart D, Delhomme B, Oheim M, Ricard C : Front Neuroanat, 2022 - Optometry for a short-sighted microscope.
Julien C, Oheim M : Biophys J, 2021 - A role for BDNF- and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity.
Khamsing D, Lebrun S, Fanget I, Larochette N, Tourain C, de Sars V, Brunstein M, Oheim M, Carrel D, Darchen F, Desnos C : Mol Brain, 2021 - A simple, inexpensive and multi-scale 3-D fluorescent test sample for optical sectioning microscopies.
Olevsko I, Szederkenyi K, Corridon J, Au A, Delhomme B, Bastien T, Fernandes J, Yip C, Oheim M, Salomon A : Microsc Res Tech, 2021 - Astrocytes respond to a neurotoxic Aβ fragment with state-dependent Ca alteration and multiphasic transmitter release.
Pham C, Hérault K, Oheim M, Maldera S, Vialou V, Cauli B, Li D : Acta Neuropathol Commun, 2021 - Infrared Excitation Induces Heating and Calcium Microdomain Hyperactivity in Cortical Astrocytes.
Schmidt E, Oheim M : Biophys J, 2020 - Infrared excitation induces brain heating and calcium microdomain hyperactivity in cortical astrocytes.
Schmidt E, Oheim M : Biophys J, 2020 - Cathodoluminescence Nanoscopy of 3D Plasmonic Networks
Racheli Ron, Marcin Stefan Zielinski, Adi Salomon : Nano Lett, 2020 - Supercritical angle fluorescence microscopy and spectroscopy (PDF)
Oheim M, Salomon A, Brunstein M : Biophys J, 2020 - Fabrication of dipole-aligned thin films of Porphyrin J-aggregates over large surfaces (PDF)
Adam Weissman, Hodaya Klimovsky, Dor Harel, Racheli Ron, Martin Oheim, Adi Salomon : Langmuir, 2020 - Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.
Rakotoson I, Delhomme B, Djian P, Deeg A, Brunstein M, Seebacher C, Uhl R, Ricard C, Oheim M : Front Neuroanat, 2019 - Calibrating evanescent-wave penetration depths for biological TIRF microscopy (PDF)
Martin Oheim, Adi Salomon, Adam Weissman, Maia Brunstein, Ute Becherer : Biophys J, 2019 - Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope
Irina Rakotoson, Brigitte Delhomme, Philippe Djian, Andreas Deeg, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clement Ricard, Martin Oheim : Front Neuroanat, 2019
Newspaper article
- Editorial: Methods and applications in frontiers in neuroanatomy
Clément Ricard, Lidia Alonso-Nanclares, Basilis Zikopoulos, Martin Oheim : Front. Neuroanat., 2023
Speech communications
- Colour-coded nanoscale calibration and optical quantification of axial fluorophore position
lya Olevsko-Arad, Moshe Feldberg, Martin Oheim, Adi Salomon : European Optical Society Annual Meeting, Dijon, 2023 - Fast, large-field fluorescence and second-harmonic generation imaging with a single-spinning disk two-photon microscope
Andreas Deeg, Federico Trigo, Doriane Hazart, Brigitte Delhomme, Tchyia Zar, Thomas Naiser, Christian Seebacher, Adi Salomon, Clément Ricard, Rainer Uhl, Martin Oheim : European Optical Society Annual Meeting, Dijon, 2023 - Nanostructured substrates for super-resolution imaging (proceeding)
Maia Brunstein, Anne Talneau, Minh-Chau Nguyen, Pascal Berto, Anne-Laure Fehrembach, Anne Sentenac, Martin Oheim : Imaging Systems and Applications 2019, Munich Germany, 2019
Patents
- Composition and method for clearing biological samples
Brigitte delhomme, Martin Oheim : Patent # : WO2024089224A1, 2024 - Calibration standard for evanescence microscopy
Martin Oheim, Adi Salomon : Patent # : WO2020053367A1, 2020